Interesting Piece! Ebola Survivors May Be the Key To Future Treatments — For Almost Any Disease

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LINA MOSES SENSED the ghost of Ebola as soon as her Land Cruiser entered the gate at Kenema Government Hospital. More than a hundred people had died in the treatment center here, an epicenter of the epidemic in Sierra Leone. A doctor who had treated them was buried on a hill overlooking the compound. When Ebola erupted in Kenema in May 2014, Moses was working here as an epidemiologist. She had never seen an Ebola patient. She could have fled home to New Orleans. Instead she stayed, fighting the outbreak and watching patients and friends die one by one.

Eventually Moses returned to the US. But now, two months later, she and one of the people she’d worked with, a physician named John Schieffelin, were back. Moses’ driver eased the Land Cruiser up to her old lab, a single-story building tucked in the corner of the hospital compound. Workers appeared and started to help unload supplies. Moses, meanwhile, stepped out into the searing midday heat and stretched her legs. She saw six people sitting on the concrete steps of an office across from her lab. Some had been nurses and researchers at Kenema; a couple were part of a newly formed survivors’ union. That’s how they’d heard about Moses’ mission.

All six had been infected with Ebola and survived. Hypothetically, that made them immune to the disease. That’s why Moses had returned—to harness that immunity to try to ensure Ebola never killed anyone again.

LINA MOSES | An epidemiologist working in Sierra Leone, Moses was one of the first Western researchers to encounter the outbreak. She later returned to find a way to fight it.
LINA MOSES | An epidemiologist working in Sierra Leone, Moses was one of the first Western researchers to encounter the outbreak. She later returned to find a way to fight it. DAYMON GARDNER

After getting set up, Moses beckoned the survivors into the lab. A technician slid needles into their veins. The survivors’ blood flowed dark red into purple-topped tubes. Moses watched in silence. Once that fluid had been a mortal danger; now it was a valuable commodity.

When the blood collection was over, Schieffelin passed a survivor outside who didn’t recognize his doctor. Schieffelin covered most of his face with his hand, imitating the mask he’d worn in the wards. “Do you remember me now?” he asked, smiling behind his palm.

Later, Moses’ boss, a virologist named Robert Garry, separated the cells they needed from the blood, washed them, and added a pink buffering liquid to each tube. Garry printed the date—January 12—and an ID number on each tube, then put the tubes into a Mr. Frosty-brand insulated container. Mr. Frosty, in turn, went into a portable freezer. Tucked safely inside, the samples chilled over the next four hours; it was crucial that they cooled slowly, so ice crystals wouldn’t destroy the cells.

Finally, at 11 that night, Moses and Garry donned purple disposable gloves, popped open the lid on Mr. Frosty, and loaded the little labeled tubes into metal cases cooled with liquid nitrogen. She handled each tube for no more than a few seconds. Even the tiny bit of heat from her fingers could warm the cells inside enough to kill them and destroy the knowledge they contained. She shut the case, ready for a journey to the United States.

Past Ebola outbreaks killed as many as 90 percent of the people who got the disease. This most recent one did not—as many as 60 percent of infected people survived. Nobody is sure why. It might have had something to do with the particular strain of the germ; for example few people bled from their eyeballs this time. Or maybe it had to do with the better standard of care many of the infected received. Regardless, thousands of people got sick but didn’t die. By definition their immune systems now make antibodies to the virus, proteins that can fight Ebola and win. Those antibodies are, essentially, the ideal medicine. Or rather they would be, if someone could unpack the biochemical manufacturing process that creates them.

Those cells Moses had collected contained the key, the blueprint for making that hypothetical drug. Scientists at Tulane University in New Orleans were waiting to try to do just that. And if they succeed? They might unlock not only a new treatment for Ebola but also a way to make new treatments for any virus, a broad-spectrum method for making drugs against diseases both common and rare, from influenza to Lassa fever. It would be a potent treatment option where today next to none exist.

Moses had tried this once before. In November she had collected another set of samples, but paperwork delays grounded the shipment in Sierra Leone. The cells thawed and died.

Now she was back at Kenema, back at the hospital where she had seen so many people die, to try again. Packed in liquid nitrogen, the blood would last 14 days—and it had to get safely back to the US. Moses had to get it onto a flight out of the airport near Freetown, the capital, by the day after tomorrow. But first Moses would have to get the samples through a countryside ripped apart by a biological apocalypse. The clock was ticking.

A soldier points an infrared thermometer at a woman in order to detect any signs of fever, one of the symptoms of Ebola, at a check point in Nikabo, a village in Kenema, Sierra Leone, on August 27, 2014.
A soldier points an infrared thermometer at a woman in order to detect any signs of fever, one of the symptoms of Ebola, at a check point in Nikabo, a village in Kenema, Sierra Leone, on August 27, 2014. MOHAMMED ELSHAMY/ANADOLU AGENCY/GETTY IMAGES

TWO DAYS LATER, Moses woke at 7 am, drank a glass of water for breakfast, and headed back to the lab. Today was transit day, and she still needed to print shipping labels and get money to pay for her driver and gas. Simbirie Jalloh, the logistics specialist at Kenema, had arranged for someone to deliver the money to Moses. That person was nowhere to be found. Jalloh was away at an Ebola task force meeting, and the only working printer was in Jalloh’s office. Which was locked.

Moses paced around her transportation, a Toyota Prado SUV, as the town woke up. Car horns chattered on the road outside the hospital’s front gate. Women with babies lashed to their backs hurried down gravel-strewn paths to appointments alongside hawkers selling cell phone chargers and sweet cakes from plastic tubs. Moses had hoped to be on the road by now; she had to arrive in Freetown by 11:30 am to get her samples onto a ferry across Tagrin Bay to the international airport in Lungi. From there they’d catch a flight to Brussels that night, and then another to Chicago and then finally New Orleans. Freetown and Lungi are only about 200 miles northwest of Kenema, but that still meant a four-hour drive on a good day. The epidemic was still raging through Sierra Leone, so there were Ebola checkpoints along the way, where officials tested everyone for fever.

The morning dragged on; Ensah, the facilities guy, arrived at 8 and let Moses into Jalloh’s office. Moses sweated as she printed the shipping labels—it was already 80 degrees—and affixed them to the two bullet-shaped dry shippers with packing tape. But she still didn’t have the money.

By 8:30, Moses was panicking. Her driver, John Sesay, was sitting on a bench under a palm tree. He calls her Dr. Moses—though she’s a doctor of nothing practical—and she calls him Dr. Sesay, a doctor of driving. But Moses was in no mood for jokes. “I can’t wait for the money,” she snapped. “John, let’s go.”
Sesay jumped up, surprised: He usually got paid first thing in the morning. But he could tell Moses was on edge and didn’t ask any questions. “Yes, Dr. Moses,” he said. Moses loaded the shippers, each 25 pounds of coolant and metal about the size of a party keg, into the rear of the SUV.

Geared up, the Toyota pulled out of the hospital gates with 30 blood samples—from the six who’d been waiting for Moses in the Kenema courtyard and 24 more—all frozen in liquid nitrogen. Moses dialed a friend in Bo, the next town along the road, and asked for $400, borrowed from money Moses herself had lent to pay for kids’ school fees.

Forty minutes later, Sesay pulled into a gas station just outside Bo, where Moses’ friend was waiting with a bag of cash. But the pumps were out of diesel. So, too, were the next two stations they passed. They saw a man on the roadside selling fuel from water bottles, and Moses thought about it. The whole town might be out. But it would take forever to fill the tank from the little 1.5-liter containers. With an eighth of a tank left, they decided to check one more station, a run-down shop in the center of town.

They were in luck—the attendant gassed up the Toyota, Moses paid him, and they were on their way at last. It was almost 10 am. They had an hour and a half to go 150 miles.

BEFORE EBOLA STRUCK, Moses had been in Sierra Leone for five years, finishing her dissertation for Tulane and working with a consortium of researchers and institutions studying viral hemorrhagic fevers. At Kenema, Moses led a team of people doing surveillance on the prevalence and spread of Lassa, a fatal disease that’s common in Sierra Leone but that most people in the West have never heard of.

In late March 2014, Moses’ network started getting reports that a different viral hemorrhagic fever had crossed from Guinea into Sierra Leone: Ebola. She sent a couple of surveillance officers to check out the reports at a place called Buedu, but they came back having found no sign of the disease. Moses and a Kenema doctor, Sheik Humarr Khan, were trying to figure out what the field team had missed when they realized that there were probably a lot of villages with similar names. Khan pulled out a map and almost immediately they spotted a village called Boidu, 30 miles northwest of where they’d sent their surveillance guys. It was on the border between Sierra Leone and Guinea. “Lina, I think you should go there,” Khan said.

After two days on dirt roads, Moses and a team got to Boidu. When she arrived, she saw a fresh pile of red dirt behind one of the houses: a grave. Her team started to ask the locals questions. Yes, a man had been buried here; yes, his son had died too, after helping him. The villagers said they had washed the dead men carefully before burial—as was customary.

Moses and the team pressed. Who cared for the men? Who touched their bodies? The villagers started to pull back. “You could see them start to realize, ‘Oh shit, we did something wrong,’” Moses says. They started to change their stories. They said that an aunt had carried the sick son to a clinic for treatment—then said she’d had nothing to do with him. Moses hoped they’d still escape the disease somehow. But if it was Ebola, it was going to spread.

Everything about treatment centers is designed to cut worker risk
A couple of months later, the lab at Kenema received a new sample to test: blood from a sick local woman. She was positive for Ebola. So were two patients who had been admitted. Khan called the staff together. “Guys, come around,” he said. “Ebola is with us in this hospital at last.”

After that they poured in, day after day. Lassa and Ebola have some symptoms in common, including (sometimes) Grand Guignol-like bleeding, so Moses and the Kenema team thought they were prepared. But the surge overwhelmed them. Doctors set up a makeshift tent and took over a second ward to care for the newcomers. Patients were stacked three to a bed, racked with fever and pain.

Ebola is transmitted through any bodily fluid—blood, sweat, tears, semen, mucus, vomit. Kenema hospital had protective Tyvek suits, gloves, and masks on hand, but the gear was poorly distributed, and doctors and nurses started to fall ill and die themselves.

That outcome wasn’t foregone. Though their expertise hadn’t yet reached Kenema, aid groups like Doctors Without Borders were learning to treat Ebola while keeping health workers safe. They were also learning to save people who were already infected.

When the virus enters the body, it induces a total overreaction in first-responder immune cells. They send a torrent of panic signals that trigger a physiological disaster: fever, pain, vomiting, diarrhea, and—if left unchecked—death. The infection moves so fast that the body’s second phase of the immune response—making antibodies that attack the virus—never has a chance to kick in. So Doctors Without Borders clinics figured out that they could reduce Ebola’s lethality with intense supportive care: Keep patients alive long enough—with antibiotics, acetaminophen and other pain medications, vitamins, and oral or intravenous fluids—and their bodies would have time to start fighting the disease. The protocol treats dehydration and weakness and, combined with soft drinks, food, and water, helps the majority of patients survive. “There’s nothing more joyous than when someone says, ‘I’m hungry, give me rice.’ Then you know you’re going to be OK,” says physician Kirrily de Polnay, who worked with Doctors Without Borders.

In the meantime, as people get sicker the bodily fluids that carry the disease start pouring out of them in ever greater volumes. So everything about a Doctors Without Borders Ebola treatment center is designed to cut worker risk. A water system dispenses two strengths of chlorine solution through dedicated taps. Access to patients is strictly controlled; workers get to the ward through only one entrance, and they leave through a single exit where they are sprayed down with bleach. Two layers of fences separate the sick from the well by 2 meters—far enough to protect from projectile vomit. And rules govern everything: from which chlorine solution is used to wash boots (0.5 percent) or dishes (0.05 percent) to how long workers can stay inside with patients (one hour).

At the start of the outbreak, Kenema Government Hospital didn’t have any of those precautions in place. They weren’t ready. Patients were leaving the wards to lie on the sidewalks, trying to escape the heat and misery inside.

One day in June, Moses saw a nurse, Alex Moigboi, caring for patients in the Ebola ward. He was wearing completely inadequate gear: just a plastic apron and a pair of gloves over his scrubs.

“Alex, what are you doing in there?” Moses shouted.

“What else am I supposed to do?” Moigboi shouted back angrily. He hadn’t been able to find the gear.

Moses ran to her lab to get it. She told Moigboi to call her next time before charging into the ward. It didn’t do any good. Like so many of Moses’ friends, Moigboi died a few weeks later. Khan, too, got infected. He died on July 29. They buried him in a grave overlooking the lab.

Moigboi’s death hit Moses especially hard. He was such a sweet, selfless kid. He was one of the first nurses to become infected, because he was one of the most dedicated, dutifully caring for patients when everyone else was scared away by the impossibility of the task. “You think that the really good people are going to pull through—that there’s got to be some justice,” Moses says.

Eventually aid groups arrived and started implementing measures they hoped would alleviate the problems. The World Health Organization built chlorinating stations throughout the Ebola unit. Doctors Without Borders helped with water and sanitation. The Red Cross helped with screening patients.

Still more nurses got sick. In the fall of 2014, Kristian Andersen, one of Moses’ colleagues back in the US, was just getting off a phone call with a friend in Kenema’s Ebola ward. Andersen, a geneticist at the Broad Institute in Cambridge, a research center affiliated with Harvard and MIT and part of the same consortium of virus-hunters as Tulane, had helped with the diagnostic lab at Kenema. Back in Cambridge, he was using leftover diagnostic samples to study the genetic sequences of the Ebola virus. As Andersen hung up the phone, he thought, “We’ve got to do something.”

It dawned on him: The patients held the answer. If they survived, they carried antibodies that targeted the very viruses that had almost killed them. The samples he’d been working with didn’t contain antibodies, but if he could get blood from survivors, he might be able to figure out how to make the same antibodies that their immune systems had produced. It would not be easy or fast, but he couldn’t stand by while more people lost their lives—if not in this outbreak, then in the next one, or the next one after that. It was time for a new plan.

A volunteer adjusts his gloves and mask as he prepares to help bury seven Ebola victims in the Kptema graveyard in Kenema, Sierra Leone, on August 24, 2014.
A volunteer adjusts his gloves and mask as he prepares to help bury seven Ebola victims in the Kptema graveyard in Kenema, Sierra Leone, on August 24, 2014.

 

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